1jx0

X-ray diffraction
2.85Å resolution

Chalcone Isomerase--Y106F mutant

Released:
Source organism: Medicago sativa
Primary publication:
Role of hydrogen bonds in the reaction mechanism of chalcone isomerase.
Biochemistry 41 5168-76 (2002)
PMID: 11955065

Function and Biology Details

Reaction catalysed:
A chalcone = a flavanone
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone--flavanone isomerase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 23.84 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28012 (Residues: 1-222; Coverage: 100%)
Gene names: CHI-1, CHI1
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P6522
Unit cell:
a: 89.56Å b: 89.56Å c: 351.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.274 0.274 0.299
Expression system: Escherichia coli BL21(DE3)