X-ray diffraction
1.63Å resolution

Chalcone Synthase--F215S mutant

Source organism: Medicago sativa

Function and Biology Details

Reaction catalysed:
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Chalcone synthase 2 Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 42.73 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P30074 (Residues: 1-389; Coverage: 100%)
Gene name: CHS2
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P3221
Unit cell:
a: 97.805Å b: 97.805Å c: 65.726Å
α: 90° β: 90° γ: 120°
R R work R free
0.2 0.19 0.225
Expression system: Escherichia coli BL21(DE3)