› 1jty

X-ray diffraction
2.97Å resolution

Crystal structure of the multidrug binding transcriptional regulator QacR bound to ethidium

Released:
DOI: 10.2210/pdb1jty/pdb
PDB 1jty coloured by chain and viewed from the front.
PDB 1jty coloured by chain and viewed from the side.
PDB 1jty coloured by chain and viewed from the top.
Source organism: Staphylococcus aureus
Primary publication:
Structural mechanisms of QacR induction and multidrug recognition.
Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG
Science 294 2158-63 (2001)
PMID: 11739955

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator QacR Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 194 amino acids
Theoretical weight: 22.98 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0N4 (Residues: 1-188; Coverage: 100%)
Gene names: SAP100B_005, SAP104C_022, qacR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 17 x SO4
Ligand ET 1 x ET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P42212
Unit cell:
a: 172Å b: 172Å c: 95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.291
Expression system: Escherichia coli

Quick links

Citations

47 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
46 more

4 mentions without citation

The TetR family of transcriptional repressors.
Ramos et al. (2005)
3 more