1jth

X-ray diffraction
2Å resolution

Crystal structure and biophysical properties of a complex between the N-terminal region of SNAP25 and the SNARE region of syntaxin 1a

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Synaptosomal-associated protein 25 Chains: A, C
Molecule details ›
Chains: A, C
Length: 82 amino acids
Theoretical weight: 9.53 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P60881 (Residues: 1-82; Coverage: 40%)
Gene names: Snap, Snap25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-1A Chains: B, D
Molecule details ›
Chains: B, D
Length: 77 amino acids
Theoretical weight: 9.1 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 191-267; Coverage: 27%)
Gene names: Sap, Stx1a
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 59.4Å b: 65.78Å c: 80.1Å
α: 90° β: 99.5° γ: 90°
R-values:
R R work R free
0.266 0.266 0.289
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli