1jtg

X-ray diffraction
1.73Å resolution

CRYSTAL STRUCTURE OF TEM-1 BETA-LACTAMASE / BETA-LACTAMASE INHIBITOR PROTEIN COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-lactamase TEM Chains: A, C
Molecule details ›
Chains: A, C
Length: 263 amino acids
Theoretical weight: 28.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P62593 (Residues: 24-286; Coverage: 100%)
Gene names: bla, blaT-3, blaT-4, blaT-5, blaT-6
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily
Beta-lactamase inhibitory protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 165 amino acids
Theoretical weight: 17.56 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli
UniProt:
  • Canonical: P35804 (Residues: 37-201; Coverage: 100%)
Sequence domains: Beta-lactamase inhibitor (BLIP)
Structure domains: Beta-lactamase Inhibitory Protein; Chain:B, domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 45.8Å b: 126.3Å c: 158.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.205
Expression system: Escherichia coli