1jql

X-ray diffraction
2.5Å resolution

Mechanism of Processivity Clamp Opening by the Delta Subunit Wrench of the Clamp Loader Complex of E. coli DNA Polymerase III: Structure of beta-delta (1-140)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta sliding clamp Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 40.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Structure domains: DNA Polymerase III, subunit A, domain 2
DNA polymerase III subunit delta Chain: B
Molecule details ›
Chain: B
Length: 140 amino acids
Theoretical weight: 15.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P28630 (Residues: 1-140; Coverage: 41%)
Gene names: JW0635, b0640, holA
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P321
Unit cell:
a: 110.1Å b: 110.1Å c: 134.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.249 0.294
Expression system: Escherichia coli