X-ray diffraction
3Å resolution

HCMV protease dimer-interface mutant, S225Y complexed to Inhibitor BILC 408


Function and Biology Details

Reaction catalysed:
Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Assemblin Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 28.21 KDa
Source organism: Human betaherpesvirus 5
Expression system: Escherichia coli
  • Canonical: P16753 (Residues: 1-256; Coverage: 36%)
Gene names: APNG, UL80
Structure domains: Herpesvirus/Caudovirus protease domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P41212
Unit cell:
a: 74.25Å b: 74.25Å c: 215.8Å
α: 90° β: 90° γ: 90°
R R work R free
0.28 0.26 0.339
Expression system: Escherichia coli