› 1jpa

X-ray diffraction
1.91Å resolution

Crystal Structure of unphosphorylated EphB2 receptor tyrosine kinase and juxtamembrane region

Released:
DOI: 10.2210/pdb1jpa/pdb
PDB entry 1jpa coloured by chain, front view.
PDB entry 1jpa coloured by chain, side view.
PDB entry 1jpa coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for autoinhibition of the Ephb2 receptor tyrosine kinase by the unphosphorylated juxtamembrane region.
Wybenga-Groot LE, Baskin B, Ong SH, Tong J, Pawson T, Sicheri F
Cell 106 745-57 (2001)
PMID: 11572780

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EphB2/CTF2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 35.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P54763 (Residues: 587-898; Coverage: 32%)
Gene names: Ephb2, Epth3, Nuk, Sek3
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P1
Unit cell:
a: 47.052Å b: 57.616Å c: 67.742Å
α: 112.95° β: 103.17° γ: 91.58°
R-values:
R R work R free
0.231 0.231 0.27
Expression system: Escherichia coli BL21

Quick links

Citations

69 review citations

Cell signaling by receptor tyrosine kinases.
Lemmon et al. (2010)
68 more

11 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
10 more