› 1jp3

X-ray diffraction
1.8Å resolution

Structure of E.coli undecaprenyl pyrophosphate synthase

Released:
DOI: 10.2210/pdb1jp3/pdb
PDB entry 1jp3 coloured by chain, front view.
PDB entry 1jp3 coloured by chain, side view.
PDB entry 1jp3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mechanism of product chain length determination and the role of a flexible loop in Escherichia coli undecaprenyl-pyrophosphate synthase catalysis.
Ko TP, Chen YK, Robinson H, Tsai PC, Gao YG, Chen AP, Wang AH, Liang PH
J Biol Chem 276 47474-82 (2001)
PMID: 11581264

Function and Biology Details

Reaction catalysed: 
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = 8 diphosphate + di-trans,octa-cis-undecaprenyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific) Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 28.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60472 (Residues: 1-253; Coverage: 100%)
Gene names: JW0169, b0174, ispU, rth, uppS, yaeS
Sequence domains: Putative undecaprenyl diphosphate synthase
Structure domains: Decaprenyl diphosphate synthase-like

Ligands and Environments

1 bound ligand:
Ligand EGC 1 x EGC
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 64.18Å b: 67.334Å c: 110.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.228
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Structure, mechanism and function of prenyltransferases.
Liang et al. (2002)
13 more

7 mentions without citation

Protein subunit interfaces: heterodimers versus homodimers.
Zhanhua et al. (2005)
6 more