› 1jnq

X-ray diffraction
2.1Å resolution

LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH EPIGALLOCATHECHIN (EGC)

Released:
DOI: 10.2210/pdb1jnq/pdb
PDB entry 1jnq coloured by chain, front view.
PDB entry 1jnq coloured by chain, side view.
PDB entry 1jnq coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Inhibition of lipoxygenase by (-)-epigallocatechin gallate: X-ray analysis at 2.1 A reveals degradation of EGCG and shows soybean LOX-3 complex with EGC instead.
Skrzypczak-Jankun E, Zhou K, Jankun J
Int J Mol Med 12 415-20 (2003)
PMID: 12964012

Function and Biology Details

Reaction catalysed: 
Linoleate + O(2) = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed linoleate 9S-lipoxygenase-3 Chain: A
Molecule details ›
Chain: A
Length: 857 amino acids
Theoretical weight: 96.92 KDa
Source organism: Glycine max
UniProt:
  • Canonical: P09186 (Residues: 1-857; Coverage: 100%)
Gene names: LOX1.3, LOX3
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FE2 1 x FE2
Ligand EGT 1 x EGT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 112.73Å b: 137.27Å c: 61.88Å
α: 90° β: 95.53° γ: 90°
R-values:
R R work R free
0.256 0.199 0.295

Quick links

Citations

4 review citations

Myostatin and the skeletal muscle atrophy and hypertrophy signaling pathways.
Rodriguez et al. (2014)
3 more

18 mentions without citation

The structural basis for specificity in lipoxygenase catalysis.
Newcomer et al. (2015)
17 more