› 1jhz

X-ray diffraction
2.4Å resolution

Purine Repressor Mutant Corepressor Binding Domain Structure

Released:
DOI: 10.2210/pdb1jhz/pdb
PDB 1jhz coloured by chain and viewed from the front.
PDB 1jhz coloured by chain and viewed from the side.
PDB 1jhz coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Role of residue 147 in the gene regulatory function of the Escherichia coli purine repressor.
Huffman JL, Lu F, Zalkin H, Brennan RG
Biochemistry 41 511-20 (2002)
PMID: 11781089

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional repressor PurR Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 32.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACP7 (Residues: 53-341; Coverage: 85%)
Gene names: JW1650, b1658, purR
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 38.02Å b: 126.13Å c: 61.84Å
α: 90° β: 99.71° γ: 90°
R-values:
R R work R free
0.178 not available not available
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Allostery in the LacI/GalR family: variations on a theme.
Swint-Kruse et al. (2009)
5 other articles

1 mention without citation

Automated selection of positions determining functional specificity of proteins by comparative analysis of orthologous groups in protein families.
Kalinina et al. (2004)