› 1jhq

X-ray diffraction
2Å resolution

Three-dimensional Structure of CobT in Complex with Reaction Products of 5-methoxybenzimidazole and NaMN

Released:
DOI: 10.2210/pdb1jhq/pdb
PDB entry 1jhq coloured by chain, front view.
PDB entry 1jhq coloured by chain, side view.
PDB entry 1jhq coloured by chain, top view.
Source organism: Salmonella enterica
Primary publication:
Structural investigation of the biosynthesis of alternative lower ligands for cobamides by nicotinate mononucleotide: 5,6-dimethylbenzimidazole phosphoribosyltransferase from Salmonella enterica.
Cheong CG, Escalante-Semerena JC, Rayment I
J Biol Chem 276 37612-20 (2001)
PMID: 11441022

Function and Biology Details

Reaction catalysed: 
Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 36.68 KDa
Source organism: Salmonella enterica
Expression system: Salmonella enterica
UniProt:
  • Canonical: Q05603 (Residues: 1-356; Coverage: 100%)
Gene names: STM2016, cobT
Sequence domains: Phosphoribosyltransferase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PMO 1 x PMO
Ligand NIO 1 x NIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 71.75Å b: 89.97Å c: 47.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.279
Expression system: Salmonella enterica

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Citations

2 review citations

Conversion of cobinamide into adenosylcobamide in bacteria and archaea.
Escalante-Semerena JC. (2007)
1 more