› 1jhh

X-ray diffraction
2.1Å resolution

LEXA S119A MUTANT

Released:
DOI: 10.2210/pdb1jhh/pdb
PDB 1jhh coloured by chain and viewed from the front.
PDB 1jhh coloured by chain and viewed from the side.
PDB 1jhh coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Crystal structure of LexA: a conformational switch for regulation of self-cleavage.
Luo Y, Pfuetzner RA, Mosimann S, Paetzel M, Frey EA, Cherney M, Kim B, Little JW, Strynadka NC
Cell 106 585-94 (2001)
PMID: 11551506

Function and Biology Details

Reaction catalysed: 
Hydrolysis of Ala(84)-|-Gly bond in repressor LexA.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LexA repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 22.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7C2 (Residues: 1-202; Coverage: 100%)
Gene names: JW4003, b4043, exrA, lexA, spr, tsl, umuA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P43212
Unit cell:
a: 89.75Å b: 89.75Å c: 102.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.273
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Targeting evolution to inhibit antibiotic resistance.
Merrikh et al. (2020)
9 more

6 mentions without citation

Structure of the LexA-DNA complex and implications for SOS box measurement.
Zhang et al. (2010)
5 more