Structure analysis

The Path of Messenger RNA Through the Ribosome. THIS FILE, 1JGP, CONTAINS THE 30S RIBOSOME SUBUNIT, THREE TRNA, AND MRNA MOLECULES. 50S RIBOSOME SUBUNIT IS IN THE FILE 1GIY

X-ray diffraction
7Å resolution
Source organism: Thermus thermophilus
Assembly composition:
hetero 25-mer (preferred)
Entry contents: 20 distinct polypeptide molecules
4 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 25-mer
Accessible surface area: 37626.79 Å2
Buried surface area: 16816.09 Å2
Dissociation area: 174.85 Å2
Dissociation energy (ΔGdiss): -21.84 kcal/mol
Dissociation entropy (TΔSdiss): 26.14 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-215874

Macromolecules

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Chain: K
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Pfam: Ribosomal protein S8
InterPro:
SCOP: Ribosome complexes

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Chain: O
Length: 135 amino acids
Theoretical weight: 14.92 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:
SCOP: Ribosome complexes

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Chain: R
Length: 89 amino acids
Theoretical weight: 10.58 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:
SCOP: Ribosome complexes

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Chain: S
Length: 91 amino acids
Theoretical weight: 10.81 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-17, 18-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:
SCOP: Ribosome complexes

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Chain: T
Length: 105 amino acids
Theoretical weight: 12.32 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P0DOY7 (Residues: 1-105; Coverage: 100%)
Gene names: TTHA1683, rpsQ
Pfam: Ribosomal protein S17
InterPro:
SCOP: Ribosome complexes

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Chain: U
Length: 88 amino acids
Theoretical weight: 10.24 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
InterPro:
SCOP: Ribosome complexes

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Chain: W
Length: 106 amino acids
Theoretical weight: 11.72 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
InterPro:
SCOP: Ribosome complexes

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Chain: X
Length: 26 amino acids
Theoretical weight: 3.22 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SIH3 (Residues: 2-27; Coverage: 96%)
Gene names: TTHA1396, rpsU
Pfam: 30S ribosomal protein Thx
InterPro:
SCOP: Ribosome complexes

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Name: 16S ribosomal RNA
Representative chains: A
Length: 1522 nucleotides
Theoretical weight: 493.96 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: tRNA(Phe)
Representative chains: B, C
Length: 76 nucleotides
Theoretical weight: 24.89 KDa
Rfam: tRNA

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Name: tRNA(Phe)
Representative chains: D
Length: 74 nucleotides
Theoretical weight: 23.73 KDa
Rfam: tRNA

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Name: MESSENGER RNA MV36
Representative chains: 1
Length: 36 nucleotides
Theoretical weight: 11.59 KDa

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