› 1jg9

X-ray diffraction
1.66Å resolution

Crystal Structure of Amylosucrase from Neisseria polysaccharea in Complex with D-glucose

Released:
DOI: 10.2210/pdb1jg9/pdb
PDB entry 1jg9 coloured by chain, front view.
PDB entry 1jg9 coloured by chain, side view.
PDB entry 1jg9 coloured by chain, top view.
Source organism: Neisseria polysaccharea
Primary publication:
Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose.
Mirza O, Skov LK, Remaud-Simeon M, Potocki de Montalk G, Albenne C, Monsan P, Gajhede M
Biochemistry 40 9032-9 (2001)
PMID: 11467966

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->4)-alpha-D-glucosyl)(n) = D-fructose + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amylosucrase Chain: A
Molecule details ›
Chain: A
Length: 628 amino acids
Theoretical weight: 71.56 KDa
Source organism: Neisseria polysaccharea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZEU2 (Residues: 13-636; Coverage: 98%)
Gene name: ams
Sequence domains: Alpha amylase, catalytic domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GLC 3 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 96.131Å b: 116.733Å c: 61.295Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.204
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria.
van Hijum et al. (2006)
5 more

3 mentions without citation

Defining structural and evolutionary modules in proteins: a community detection approach to explore sub-domain architecture.
Hleap et al. (2013)
2 more