X-ray diffraction
1.5Å resolution

Crystal Structure of L-isoaspartyl (D-aspartyl) O-methyltransferase with adenosine


Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Protein-L-isoaspartate O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.61 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
  • Canonical: Q8TZR3 (Residues: 1-219; Coverage: 100%)
Gene names: PF1922, pcm
Sequence domains: Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 38.201Å b: 52.522Å c: 96.129Å
α: 90° β: 90° γ: 90°
R R work R free
0.229 0.221 0.236
Expression system: Escherichia coli