Structure analysis

Crystal Structure of the NC2-TBP-DNA Ternary Complex

X-ray diffraction
2.62Å resolution
Source organism: Homo sapiens
Assembly composition:
hetero pentamer (preferred)
Entry contents: 3 distinct polypeptide molecules
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 23700 Å2
Buried surface area: 11900 Å2
Dissociation area: 1,700 Å2
Dissociation energy (ΔGdiss): 7 kcal/mol
Dissociation entropy (TΔSdiss): 13 kcal/mol
Interface energy (ΔGint): -81 kcal/mol
Symmetry number: 1

Macromolecules

Chain: A
Length: 98 amino acids
Theoretical weight: 10.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14919 (Residues: 1-77; Coverage: 38%)
Gene name: DRAP1
Pfam: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
InterPro:
CATH: Histone, subunit A
SCOP: TBP-associated factors, TAFs

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Chain: B
Length: 179 amino acids
Theoretical weight: 19.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01658 (Residues: 1-176; Coverage: 100%)
Gene name: DR1
Pfam: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
InterPro:
CATH: Histone, subunit A
SCOP: TBP-associated factors, TAFs

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Chain: C
Length: 185 amino acids
Theoretical weight: 20.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20226 (Residues: 159-339; Coverage: 53%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Pfam: Transcription factor TFIID (or TATA-binding protein, TBP)
InterPro:
CATH: TATA-Binding Protein
SCOP: TATA-box binding protein (TBP), C-terminal domain

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Name: 5'-D(*TP*TP*GP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*TP*CP*C)-3'
Representative chains: D
Length: 19 nucleotides
Theoretical weight: 5.84 KDa

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Name: 5'-D(*G*GP*AP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*CP*AP*A)-3'
Representative chains: E
Length: 19 nucleotides
Theoretical weight: 5.8 KDa

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