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X-ray diffraction
1Å resolution

X-ray structure of nitric oxide reductase (cytochrome P450nor) in the ferric resting state at atomic resolution

Released:
Source organism: Fusarium oxysporum
Primary publication:
X-ray structure of nitric oxide reductase (cytochrome P450nor) at atomic resolution.
Acta Crystallogr. D Biol. Crystallogr. 58 81-9 (2002)
PMID: 11752781

Function and Biology Details

Reaction catalysed:
Nitrous oxide + NAD(P)(+) + H(2)O = 2 nitric oxide + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP nitrous oxide-forming nitric oxide reductase Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 44.52 KDa
Source organism: Fusarium oxysporum
Expression system: Escherichia coli
UniProt:
  • Canonical: P23295 (Residues: 1-403; Coverage: 100%)
Gene names: CYP55, CYP55A1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 54.661Å b: 82.132Å c: 85.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.102 0.102 0.139
Expression system: Escherichia coli