› 1jbu

X-ray diffraction
2Å resolution

Coagulation Factor VII Zymogen (EGF2/Protease) in Complex with Inhibitory Exosite Peptide A-183

Released:
DOI: 10.2210/pdb1jbu/pdb
PDB 1jbu coloured by chain and viewed from the front.
PDB 1jbu coloured by chain and viewed from the side.
PDB 1jbu coloured by chain and viewed from the top.
Source organisms:
Primary publication:
The factor VII zymogen structure reveals reregistration of beta strands during activation.
Eigenbrot C, Kirchhofer D, Dennis MS, Santell L, Lazarus RA, Stamos J, Ultsch MH
Structure 9 627-36 (2001)
PMID: 11470437

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Factor VII heavy chain Chain: H
Molecule details ›
Chain: H
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Factor VII light chain Chain: L
Molecule details ›
Chain: L
Length: 63 amino acids
Theoretical weight: 6.87 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08709 (Residues: 150-212; Coverage: 14%)
Gene name: F7
Structure domains: Laminin
Peptide exosite inhibitor A-183 Chain: X
Molecule details ›
Chain: X
Length: 15 amino acids
Theoretical weight: 2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand BEN 1 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 67.44Å b: 84.51Å c: 84.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.204 0.227
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

10 review citations

Discovery methodology for the development of direct factor VIIa inhibitors.
Henry et al. (2014)
9 more

9 mentions without citation

Mechanisms of macromolecular protease inhibitors.
Farady et al. (2010)
8 more