1jb7

X-ray diffraction
1.86Å resolution

DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Telomere-binding protein subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 56.17 KDa
Source organism: Sterkiella nova
Expression system: Escherichia coli
UniProt:
  • Canonical: P29549 (Residues: 1-495; Coverage: 100%)
Gene names: MAC-56A, MAC-56K, MAC-56S
Sequence domains: Telomeric single stranded DNA binding POT1/CDC13
Structure domains: Nucleic acid-binding proteins
Telomere-binding protein subunit beta Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 28.72 KDa
Source organism: Sterkiella nova
Expression system: Escherichia coli
UniProt:
  • Canonical: P16458 (Residues: 1-260; Coverage: 68%)
Gene names: MAC-41A, MAC-41S
Structure domains: Telomere-binding Protein Beta Subunit; Chain
5'-D(*GP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*G)-3' Chains: D, G, H
Molecule details ›
Chains: D, G, H
Length: 12 nucleotides
Theoretical weight: 3.81 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6122
Unit cell:
a: 93.1Å b: 93.1Å c: 421.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.23 0.246
Expression system: Escherichia coli