› 1j9e

X-ray diffraction
1.44Å resolution

Low Temperature (100K) Crystal Structure of Flavodoxin D. vulgaris S35C Mutant at 1.44 Angstrom Resolution

Released:
DOI: 10.2210/pdb1j9e/pdb
PDB 1j9e coloured by chain and viewed from the front.
PDB 1j9e coloured by chain and viewed from the side.
PDB 1j9e coloured by chain and viewed from the top.
Source organism: Desulfovibrio vulgaris
Primary publication:
Comparison of the refined crystal structures of wild-type (1.34 A) flavodoxin from Desulfovibrio vulgaris and the S35C mutant (1.44 A) at 100 K.
Artali R, Bombieri G, Meneghetti F, Gilardi G, Sadeghi SJ, Cavazzini D, Rossi GL
Acta Crystallogr. D Biol. Crystallogr. 58 1787-92 (2002)
PMID: 12351822

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 15.69 KDa
Source organism: Desulfovibrio vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: P00323 (Residues: 3-148; Coverage: 99%)
Gene name: DVU_2680
Sequence domains: Flavodoxin
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P43212
Unit cell:
a: 50.55Å b: 50.55Å c: 138.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.136 0.156
Expression system: Escherichia coli

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Citations

7 citation in other articles

A crystallographic study of Cys69Ala flavodoxin II from Azotobacter vinelandii: structural determinants of redox potential.
Alagaratnam et al. (2005)
6 more