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Structural basis of the interaction of the pyelonephritic E. coli adhesin to its human kidney receptor.

Dodson KW, Pinkner JS, Rose T, Magnusson G, Hultgren SJ, Waksman G
Cell 105 733-43 (2001)
Cited: 148 times
EuropePMC logo PMID: 11440716

Abstract

PapG is the adhesin at the tip of the P pilus that mediates attachment of uropathogenic Escherichia coli to the uroepithelium of the human kidney. The human specific allele of PapG binds to globoside (GbO4), which consists of the tetrasaccharide GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc linked to ceramide. Here, we present the crystal structures of a binary complex of the PapG receptor binding domain bound to GbO4 as well as the unbound form of the adhesin. The biological importance of each of the residues involved in binding was investigated by site-directed mutagenesis. These studies provide a molecular snapshot of a host-pathogen interaction that determines the tropism of uropathogenic E. coli for the human kidney and is critical to the pathogenesis of pyelonephritis.

Reviews - 1j8r mentioned but not cited (5)

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