› 1j59

X-ray diffraction
2.5Å resolution

CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb1j59/pdb
PDB 1j59 coloured by chain and viewed from the front.
PDB 1j59 coloured by chain and viewed from the side.
PDB 1j59 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Entry authors: Parkinson G, Wilson C, Gunasekera A, Ebright YW, Ebright RH, Berman HM

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
cAMP-activated global transcriptional regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 2-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
5'-D(*GP*CP*GP*AP*AP*AP*AP*GP*TP*GP*TP*GP*AP*C)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 14 nucleotides
Theoretical weight: 4.35 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*AP*TP*AP*TP*GP*TP*CP*AP*CP*AP*CP*TP*TP*TP*TP*CP*G )-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 17 nucleotides
Theoretical weight: 5.15 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CMP 2 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2221
Unit cell:
a: 136.99Å b: 152.8Å c: 76.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.279
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

C4-Dicarboxylates as Growth Substrates and Signaling Molecules for Commensal and Pathogenic Enteric Bacteria in Mammalian Intestine.
Schubert et al. (2022)
17 more

10 mentions without citation

Normal mode analysis as a method to derive protein dynamics information from the Protein Data Bank.
Wako et al. (2017)
9 more