1j3q

X-ray diffraction
1.85Å resolution

Crystal structure of Thermococcus litoralis phosphogrucose isomerase soaked with FeSO4

Released:
Source organism: Thermococcus litoralis
Entry authors: Jeong J-J, Fushinobu S, Ito S, Hidaka M, Shoun H, Wakagi T

Function and Biology Details

Reaction catalysed:
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.75 KDa
Source organism: Thermococcus litoralis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84140 (Residues: 1-190; Coverage: 100%)
Gene name: pgiA
Sequence domains: Glucose-6-phosphate isomerase (GPI)
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 40.52Å b: 81.07Å c: 126.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.238 0.278
Expression system: Escherichia coli BL21(DE3)