› 1j3f

X-ray diffraction
1.45Å resolution

Crystal Structure of an Artificial Metalloprotein:Cr(III)(3,3'-Me2-salophen)/apo-A71G Myoglobin

Released:
DOI: 10.2210/pdb1j3f/pdb
PDB 1j3f coloured by chain and viewed from the front.
PDB 1j3f coloured by chain and viewed from the side.
PDB 1j3f coloured by chain and viewed from the top.
Source organism: Physeter catodon
Primary publication:
Coordinated design of cofactor and active site structures in development of new protein catalysts.
Ueno T, Koshiyama T, Ohashi M, Kondo K, Kono M, Suzuki A, Yamane T, Watanabe Y
J. Am. Chem. Soc. 127 6556-62 (2005)
PMID: 15869276

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.35 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 1-154; Coverage: 100%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand CR 1 x CR
Ligand PO4 6 x PO4
Ligand CZM 1 x CZM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P212121
Unit cell:
a: 32.972Å b: 58.787Å c: 76.237Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.219
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Design of artificial metalloproteins/metalloenzymes by tuning noncovalent interactions.
Hirota et al. (2018)
19 more

2 mentions without citation

Design of functional metalloproteins.
Lu et al. (2009)
1 more