› 1j3b

X-ray diffraction
2Å resolution

Crystal structure of ATP-dependent phosphoenolpyruvate carboxykinase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1j3b/pdb
PDB entry 1j3b coloured by chain, front view.
PDB entry 1j3b coloured by chain, side view.
PDB entry 1j3b coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structure of ATP-dependent phosphoenolpyruvate carboxykinase from Thermus thermophilus HB8 showing the structural basis of induced fit and thermostability.
Sugahara M, Ohshima N, Ukita Y, Sugahara M, Kunishima N
Acta Crystallogr D Biol Crystallogr 61 1500-7 (2005)
PMID: 16239727

Function and Biology Details

Reaction catalysed: 
ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178181 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase (ATP) Chains: A, B
Molecule details ›
Chains: A, B
Length: 529 amino acids
Theoretical weight: 59.39 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLL5 (Residues: 1-529; Coverage: 100%)
Gene names: TTHA0278, pckA, tthHB8IM
Sequence domains: Phosphoenolpyruvate carboxykinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand PO4 9 x PO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 64.702Å b: 129.91Å c: 173.391Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.226
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Crystal structures of shikimate dehydrogenase AroE from Thermus thermophilus HB8 and its cofactor and substrate complexes: insights into the enzymatic mechanism.
Bagautdinov et al. (2007)
6 more