X-ray diffraction
2.2Å resolution

Crystal structure of Urate oxidase from Bacillus SP. TB-90 co-crystallized with 8-Azaxanthine

Source organism: Bacillus sp. TB-90
Entry authors: Hibi T, Nago T, Nishiya Y, Oda J

Function and Biology Details

Reactions catalysed:
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Uric acid degradation bifunctional protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 36.58 KDa
Source organism: Bacillus sp. TB-90
Expression system: Escherichia coli
  • Canonical: Q45697 (Residues: 171-489; Coverage: 64%)
Gene name: uao
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 133.39Å b: 144.41Å c: 78.785Å
α: 90° β: 90° γ: 90°
R R work R free
0.219 0.18 0.218
Expression system: Escherichia coli