X-ray diffraction
2.1Å resolution

Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with substrate


Function and Biology Details

Reaction catalysed:
ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Argininosuccinate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 400 amino acids
Theoretical weight: 44.88 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
  • Canonical: P59846 (Residues: 1-400; Coverage: 100%)
Gene names: TTHA0284, argG
Sequence domains: Arginosuccinate synthase
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: R3
Unit cell:
a: 229.33Å b: 229.33Å c: 159.26Å
α: 90° β: 90° γ: 120°
R R work R free
0.226 0.226 0.257
Expression system: Escherichia coli