› 1j0k

X-ray diffraction
3.2Å resolution

Crystal structure of neopullulanase E357Q complex with isopanose

Released:
DOI: 10.2210/pdb1j0k/pdb
PDB entry 1j0k coloured by chain, front view.
PDB entry 1j0k coloured by chain, side view.
PDB entry 1j0k coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase.
Hondoh H, Kuriki T, Matsuura Y
J Mol Biol 326 177-88 (2003)
PMID: 12547200

Function and Biology Details

Reaction catalysed: 
Hydrolysis of pullulan to panose (6-alpha-D-glucosylmaltose)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153781 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neopullulanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 588 amino acids
Theoretical weight: 69.14 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P38940 (Residues: 1-588; Coverage: 100%)
Gene name: nplT
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P21
Unit cell:
a: 68.006Å b: 73.758Å c: 123.246Å
α: 90° β: 90.15° γ: 90°
R-values:
R R work R free
0.174 0.174 0.212
Expression system: Escherichia coli

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Citations

7 review citations

The Sus operon: a model system for starch uptake by the human gut Bacteroidetes.
Foley et al. (2016)
6 more

3 mentions without citation

Novel Maltogenic Amylase CoMA from Corallococcus sp. Strain EGB Catalyzes the Conversion of Maltooligosaccharides and Soluble Starch to Maltose.
Zhou et al. (2018)
2 more