X-ray diffraction
2.5Å resolution

Crystal Structure Analysis of the ACC deaminase homologue


Function and Biology Details

Reaction catalysed:
(1a) 1-aminocyclopropane-1-carboxylate = 2-aminobut-2-enoate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Entry contents:
1 distinct polypeptide molecule
Putative 1-aminocyclopropane-1-carboxylate deaminase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 325 amino acids
Theoretical weight: 35.23 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O57809 (Residues: 1-325; Coverage: 100%)
Gene names: PH0054, PHBE027
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

Cofactor: Ligand PLP 3 x PLP
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P3221
Unit cell:
a: 122.34Å b: 122.34Å c: 115.031Å
α: 90° β: 90° γ: 120°
R R work R free
0.201 0.201 0.27
Expression system: Escherichia coli BL21(DE3)