› 1izc

X-ray diffraction
1.7Å resolution

Crystal Structure Analysis of Macrophomate synthase

Released:
DOI: 10.2210/pdb1izc/pdb
PDB 1izc coloured by chain and viewed from the front.
PDB 1izc coloured by chain and viewed from the side.
PDB 1izc coloured by chain and viewed from the top.
Source organism: Macrophoma commelinae
Primary publication:
Insight into a natural Diels-Alder reaction from the structure of macrophomate synthase.
Ose T, Watanabe K, Mie T, Honma M, Watanabe H, Yao M, Oikawa H, Tanaka I
Nature 422 185-9 (2003)
PMID: 12634789

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HpcH_HpaI domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 36.27 KDa
Source organism: Macrophoma commelinae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UVD4 (Residues: 1-339; Coverage: 100%)
Sequence domains: HpcH/HpaI aldolase/citrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand PYR 1 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P6322
Unit cell:
a: 106.678Å b: 106.678Å c: 120.755Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

The expanding world of biosynthetic pericyclases: cooperation of experiment and theory for discovery.
Jamieson et al. (2019)
15 more

2 mentions without citation

Natural [4 + 2]-Cyclases.
Jeon et al. (2017)
1 more