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X-ray diffraction
2.1Å resolution

Toho-1 beta-Lactamase In Complex With Benzylpenicillin

Released:

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Toho-1 Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 28.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47066 (Residues: 30-291; Coverage: 100%)
Gene name: bla
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P3221
Unit cell:
a: 72.799Å b: 72.799Å c: 99.467Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.227
Expression system: Escherichia coli