1ixy

X-ray diffraction
2.5Å resolution

Ternary complex of T4 phage BGT with UDP and a 13 mer DNA duplex

Released:

Function and Biology Details

Reaction catalysed:
Transfers a beta-D-glucosyl residue from UDP-alpha-D-glucose to a hydroxymethylcytosine residue in DNA
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA beta-glucosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 40.72 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04547 (Residues: 1-351; Coverage: 100%)
Gene name: bgt
Sequence domains: Bacteriophage T4 beta-glucosyltransferase
Structure domains: Glycogen Phosphorylase B;
5'-D(*GP*AP*TP*AP*CP*TP*3DRP*AP*GP*AP*TP*AP*G)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 13 nucleotides
Theoretical weight: 3.89 KDa
Source organism: Escherichia virus T4
Expression system: Not provided
5'-D(*CP*TP*AP*TP*CP*TP*GP*AP*GP*TP*AP*TP*C)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
Source organism: Escherichia virus T4
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 78.9Å b: 56.4Å c: 99.1Å
α: 90° β: 90.3° γ: 90°
R-values:
R R work R free
0.209 0.209 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided