1ixr

X-ray diffraction
3.3Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Holliday junction ATP-dependent DNA helicase RuvA Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 20.44 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9F1Q3 (Residues: 1-191; Coverage: 100%)
Gene names: TTHA0291, ruvA
Sequence domains:
Structure domains:
Holliday junction ATP-dependent DNA helicase RuvB Chain: C
Molecule details ›
Chain: C
Length: 312 amino acids
Theoretical weight: 34.8 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SL87 (Residues: 1-312; Coverage: 96%)
Gene names: TTHA0406, ruvB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: I222
Unit cell:
a: 61.581Å b: 195.113Å c: 246.244Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.268 0.335
Expression system: Escherichia coli BL21(DE3)