› 1iw8

X-ray diffraction
2.5Å resolution

Crystal Structure of a mutant of acid phosphatase from Escherichia blattae (G74D/I153T)

Released:
DOI: 10.2210/pdb1iw8/pdb
PDB entry 1iw8 coloured by chain, front view.
PDB entry 1iw8 coloured by chain, side view.
PDB entry 1iw8 coloured by chain, top view.
Source organism: Shimwellia blattae
Primary publication:
Enhancement of nucleoside phosphorylation activity in an acid phosphatase.
Ishikawa K, Mihara Y, Shimba N, Ohtsu N, Kawasaki H, Suzuki E, Asano Y
Protein Eng 15 539-43 (2002)
PMID: 12200535

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193376 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acid phosphatase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 231 amino acids
Theoretical weight: 25.08 KDa
Source organism: Shimwellia blattae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S1A6 (Residues: 19-249; Coverage: 100%)
Gene name: pho
Sequence domains: PAP2 superfamily
Structure domains: Phosphatidic acid phosphatase type 2/haloperoxidase

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P212121
Unit cell:
a: 137.8Å b: 168.2Å c: 58.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.284
Expression system: Escherichia coli

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Citations

1 review citation

Microbial innovations in the world of food.
Kawasaki et al. (2017)
6 other articles