1it8

X-ray diffraction
2.5Å resolution

Crystal structure of archaeosine tRNA-guanine transglycosylase from Pyrococcus horikoshii complexed with archaeosine precursor, preQ0

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA-guanine(15) transglycosylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 582 amino acids
Theoretical weight: 66.7 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58843 (Residues: 1-582; Coverage: 100%)
Gene names: PH1116, tgtA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45PX
Spacegroup: P43212
Unit cell:
a: 100.467Å b: 100.467Å c: 366.168Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.278
Expression system: Escherichia coli BL21(DE3)