1isp

X-ray diffraction
1.3Å resolution

Crystal structure of Bacillus subtilis lipase at 1.3A resolution

Released:
Source organism: Bacillus subtilis

Function and Biology Details

Reaction catalysed:
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase EstA Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 19.38 KDa
Source organism: Bacillus subtilis
Expression system: Bacillus megaterium
UniProt:
  • Canonical: P37957 (Residues: 32-212; Coverage: 100%)
Gene names: BSU02700, estA, lip, lipA
Sequence domains: Lipase (class 2)
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 38.2Å b: 58.45Å c: 82.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.232
Expression system: Bacillus megaterium