1is9

X-ray diffraction
1.03Å resolution

Endoglucanase A from Clostridium thermocellum at atomic resolution

Released:
Primary publication:
Advantages of high-resolution phasing: MAD to atomic resolution.
Acta Crystallogr. D Biol. Crystallogr. 58 1433-41 (2002)
PMID: 12198299

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase A Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 40.35 KDa
Source organism: Hungateiclostridium thermocellum
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DC29 (Residues: 33-395; Coverage: 82%)
Gene names: Cthe_0269, celA
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 49.441Å b: 62.829Å c: 103.891Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.131 0.149
Expression system: Escherichia coli