› 1is9

X-ray diffraction
1.03Å resolution

Endoglucanase A from Clostridium thermocellum at atomic resolution

Released:
DOI: 10.2210/pdb1is9/pdb
PDB entry 1is9 coloured by chain, front view.
PDB entry 1is9 coloured by chain, side view.
PDB entry 1is9 coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Advantages of high-resolution phasing: MAD to atomic resolution.
Schmidt A, Gonzalez A, Morris RJ, Costabel M, Alzari PM, Lamzin VS
Acta Crystallogr D Biol Crystallogr 58 1433-41 (2002)
PMID: 12198299

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107021 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase A Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 40.35 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DC29 (Residues: 33-395; Coverage: 82%)
Gene names: Cthe_0269, celA
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

2 bound ligands:
Ligand HG 6 x HG
Ligand CL 7 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 49.441Å b: 62.829Å c: 103.891Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.131 0.149
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Thermophilic lignocellulose deconstruction.
Blumer-Schuette et al. (2014)
4 other articles

3 mentions without citation

Insertion of endocellulase catalytic domains into thermostable consensus ankyrin scaffolds: effects on stability and cellulolytic activity.
Cunha et al. (2013)
2 more