› 1ino

X-ray diffraction
2.2Å resolution

RECOMBINANT INORGANIC PYROPHOSPHATASE FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1ino/pdb
PDB entry 1ino coloured by chain, front view.
PDB entry 1ino coloured by chain, side view.
PDB entry 1ino coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mg2+ activation of Escherichia coli inorganic pyrophosphatase.
Avaeva SM, Rodina EV, Kurilova SA, Nazarova TI, Vorobyeva NN, Harutyunyan EH
FEBS Lett 377 44-6 (1995)
PMID: 8543015

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7A9 (Residues: 2-176; Coverage: 99%)
Gene names: JW4185, b4226, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R32
Unit cell:
a: 110.4Å b: 110.4Å c: 76.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 not available not available
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Evolutionary aspects of inorganic pyrophosphatase.
Sivula et al. (1999)
9 more

3 mentions without citation

Physics and evolution of thermophilic adaptation.
Berezovsky et al. (2005)
2 more