1inj

X-ray diffraction
1.55Å resolution

CRYSTAL STRUCTURE OF THE APO FORM OF 4-DIPHOSPHOCYTIDYL-2-C-METHYLERYTHRITOL (CDP-ME) SYNTHETASE (YGBP) INVOLVED IN MEVALONATE INDEPENDENT ISOPRENOID BIOSYNTHESIS

Released:

Function and Biology Details

Reaction catalysed:
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 25.77 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46893 (Residues: 1-236; Coverage: 100%)
Gene names: JW2717, b2747, ispD, ygbP
Sequence domains: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 130.297Å b: 47.328Å c: 38.146Å
α: 90° β: 94.18° γ: 90°
R-values:
R R work R free
0.245 0.245 0.265
Expression system: Escherichia coli BL21(DE3)