› 1in5

X-ray diffraction
2Å resolution

THERMOGOTA MARITIMA RUVB A156S MUTANT

Released:
DOI: 10.2210/pdb1in5/pdb
PDB 1in5 coloured by chain and viewed from the front.
PDB 1in5 coloured by chain and viewed from the side.
PDB 1in5 coloured by chain and viewed from the top.
Source organism: Thermotoga maritima
Primary publication:
Structure and mechanism of the RuvB Holliday junction branch migration motor.
Putnam CD, Clancy SB, Tsuruta H, Gonzalez S, Wetmur JG, Tainer JA
J. Mol. Biol. 311 297-310 (2001)
PMID: 11478862

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction branch migration complex subunit RuvB Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 37.22 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q56313 (Residues: 1-334; Coverage: 100%)
Gene names: TM_1730, ruvB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P65
Unit cell:
a: 86.664Å b: 86.664Å c: 82.567Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.216 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

OB-fold Families of Genome Guardians: A Universal Theme Constructed From the Small β-barrel Building Block.
Bianco PR. (2022)
25 more

1 mention without citation

Crystal structure of a novel archaeal AAA+ ATPase SSO1545 from Sulfolobus solfataricus.
Xu et al. (2009)