PDBe 1im3

X-ray diffraction
2.2Å resolution

Crystal Structure of the human cytomegalovirus protein US2 bound to the MHC class I molecule HLA-A2/tax

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein Tax-1 Chains: C, G, K, O
Molecule details ›
Chains: C, G, K, O
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human T-cell lymphotrophic virus type 1 (Caribbean isolate)
Expression system: Not provided
UniProt:
  • Canonical: P14079 (Residues: 11-19; Coverage: 3%)
Gene name: tax
Unique short US2 glycoprotein Chains: D, H, L, P
Molecule details ›
Chains: D, H, L, P
Length: 95 amino acids
Theoretical weight: 10.9 KDa
Source organism: Human betaherpesvirus 5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09713 (Residues: 43-137; Coverage: 48%)
Gene name: US2
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P1
Unit cell:
a: 95.48Å b: 96.7Å c: 99.54Å
α: 109.02° β: 109.46° γ: 107.93°
R-values:
R R work R free
0.222 0.206 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided