1im0

X-ray diffraction
2.98Å resolution

OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI N156A ACTIVE SITE MUTANT PH 8.3

Released:

Function and Biology Details

Reactions catalysed:
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Phosphatidylcholine + H(2)O = 2-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A1 Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A921 (Residues: 21-175, 177-289; Coverage: 100%)
Gene names: JW3794, b3821, pldA
Sequence domains: Phospholipase A1
Structure domains: Phospholipase A1

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P3121
Unit cell:
a: 78.462Å b: 78.462Å c: 101.424Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.266
Expression system: Escherichia coli