› 1ij8

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF LITE AVIDIN-BNI COMPLEX

Released:
DOI: 10.2210/pdb1ij8/pdb
PDB entry 1ij8 coloured by chain, front view.
PDB entry 1ij8 coloured by chain, side view.
PDB entry 1ij8 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Chicken avidin exhibits pseudo-catalytic properties. Biochemical, structural, and electrostatic consequences.
Huberman T, Eisenberg-Domovich Y, Gitlin G, Kulik T, Bayer EA, Wilchek M, Livnah O
J Biol Chem 276 32031-9 (2001)
PMID: 11395489

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.35 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P02701 (Residues: 25-152; Coverage: 100%)
Gene name: AVD
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand BNI 2 x BNI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P21212
Unit cell:
a: 72.269Å b: 80.362Å c: 43.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.234

Quick links

Citations

3 review citations

Streptavidin-biotin technology: improvements and innovations in chemical and biological applications.
Dundas et al. (2013)
2 more