1ij0 Citations

Buried polar residues in coiled-coil interfaces.

Biochemistry 40 6352-60 (2001)
Related entries: 1ij1, 1ij2, 1ij3

Cited: 71 times
EuropePMC logo PMID: 11371197

Abstract

Coiled coils, estimated to constitute 3-5% of the encoded residues in most genomes, are characterized by a heptad repeat, (abcdefg)(n), where the buried a and d positions form the interface between multiple alpha-helices. Although generally hydrophobic, a substantial fraction ( approximately 20%) of these a- and d-position residues are polar or charged. We constructed variants of the well-characterized coiled coil GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a or a d position. The stability and oligomeric specificity of each variant were measured, and crystal structures of coiled-coil trimers with threonine or serine at either an a or a d position were determined. The structures show how single polar residues in the interface affect not only local packing, but also overall coiled-coil geometry as seen by changes in the Crick supercoil parameters and core cavity volumes.

Reviews citing this publication (6)

  1. De Novo Designed α-Helical Coiled-Coil Peptides as Scaffolds for Chemical Reactions. Rink WM, Thomas F. Chemistry 25 1665-1677 (2019)
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