X-ray diffraction
1.8Å resolution

Crystal Structure of UDP-glucosyltransferase GtfB


Function and Biology Details

Reaction catalysed:
UDP-alpha-D-glucose + vancomycin aglycone = UDP + devancoaminyl-vancomycin
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Vancomycin aglycone glucosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 43.78 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P96559 (Residues: 1-407; Coverage: 100%)
Gene name: gtfB
Sequence domains: Glycosyltransferase family 28 N-terminal domain
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 102.11Å b: 102.11Å c: 83.35Å
α: 90° β: 90° γ: 90°
R R work R free
0.211 0.211 0.231
Expression system: Escherichia coli BL21(DE3)