1iaw

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type-2 restriction enzyme NaeI Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.38 KDa
Source organism: Lechevalieria aerocolonigenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P50187 (Residues: 1-317; Coverage: 100%)
Gene name: naeIR
Sequence domains: Restriction endonuclease NaeI
Structure domains:
5'-D(*TP*GP*CP*CP*AP*CP*GP*CP*CP*GP*GP*CP*GP*TP*GP*GP*C)-3' Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 17 nucleotides
Theoretical weight: 5.21 KDa
Source organism: Lentzea aerocolonigenes
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 59.6Å b: 65Å c: 258.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.293
Expression systems:
  • Escherichia coli
  • Not provided