1i84

Electron crystallography
20Å resolution

CRYO-EM STRUCTURE OF THE HEAVY MEROMYOSIN SUBFRAGMENT OF CHICKEN GIZZARD SMOOTH MUSCLE MYOSIN WITH REGULATORY LIGHT CHAIN IN THE DEPHOSPHORYLATED STATE. ONLY C ALPHAS PROVIDED FOR REGULATORY LIGHT CHAIN. ONLY BACKBONE ATOMS PROVIDED FOR S2 FRAGMENT.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myosin-11 Chains: S, V
Molecule details ›
Chains: S, V
Length: 1184 amino acids
Theoretical weight: 137.07 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10587 (Residues: 2-1175; Coverage: 59%)
Gene name: MYH11
Sequence domains:
Myosin light polypeptide 6 Chains: T, W
Molecule details ›
Chains: T, W
Length: 150 amino acids
Theoretical weight: 16.87 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P02607 (Residues: 2-151; Coverage: 99%)
Gene name: MYL6
Myosin regulatory light chain 2, skeletal muscle isoform Chains: U, Z
Molecule details ›
Chains: U, Z
Length: 166 amino acids
Theoretical weight: 18.33 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P02609 (Residues: 3-168; Coverage: 99%)
Gene name: MYLPF

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 20Å
Expression system: Spodoptera frugiperda