› 1i5e

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF BACILLUS CALDOLYTICUS URACIL PHOSPHORIBOSYLTRANSFERASE WITH BOUND UMP

Released:
DOI: 10.2210/pdb1i5e/pdb
PDB entry 1i5e coloured by chain, front view.
PDB entry 1i5e coloured by chain, side view.
PDB entry 1i5e coloured by chain, top view.
Source organism: [Bacillus] caldolyticus
Primary publication:
Structure of product-bound Bacillus caldolyticus uracil phosphoribosyltransferase confirms ordered sequential substrate binding.
Kadziola A, Neuhard J, Larsen S
Acta Crystallogr D Biol Crystallogr 58 936-45 (2002)
PMID: 12037295

Function and Biology Details

Reaction catalysed: 
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 22.86 KDa
Source organism: [Bacillus] caldolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P70881 (Residues: 1-209; Coverage: 100%)
Gene name: upp
Sequence domains: Uracil phosphoribosyltransferase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand U5P 2 x U5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P3121
Unit cell:
a: 89.4Å b: 89.4Å c: 163.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.27
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors.
Turnbough et al. (2008)
1 more

2 mentions without citation

Thioredoxin Profiling of Multiple Thioredoxin-Like Proteins in Staphylococcus aureus.
Peng et al. (2018)
1 more