› 1i4j

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF L22 RIBOSOMAL PROTEIN MUTANT

Released:
DOI: 10.2210/pdb1i4j/pdb
PDB entry 1i4j coloured by chain, front view.
PDB entry 1i4j coloured by chain, side view.
PDB entry 1i4j coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
L22 ribosomal protein and effect of its mutation on ribosome resistance to erythromycin.
Davydova N, Streltsov V, Wilce M, Liljas A, Garber M
J Mol Biol 322 635-44 (2002)
PMID: 12225755

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155716 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit protein uL22 Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.35 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48286 (Residues: 1-113; Coverage: 97%)
Gene names: rpl22, rplV
Sequence domains: Ribosomal protein L22p/L17e
Structure domains: Ribosomal protein L22/L17

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 31.88Å b: 86.59Å c: 38.96Å
α: 90° β: 104.47° γ: 90°
R-values:
R R work R free
0.208 0.209 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The bacterial ribosome as a target for antibiotics.
Poehlsgaard et al. (2005)
9 more

3 mentions without citation

The extended loops of ribosomal proteins L4 and L22 are not required for ribosome assembly or L4-mediated autogenous control.
Zengel et al. (2003)
2 more